納米泡氫水具有更強的抗氧化效果

本文作者:上海第二軍醫大學 ?孫學軍教授

最近日本全州廣島大學學者Shinya Kato等在Materials Science andEngineering: C發表論文Antioxidant activities of nano-bubblehydrogen-dissolved water assessed by ESR and 2,2’-bipyridyl methods。

研究采用兩種自由基檢測方法,證明納米氣泡水制備氫氣水抗氧化效果比普通的氫氣水更明顯。研究的水包括純凈水、自來水、普通商品氫氣水、工業化氫氣水和納米氣泡氫氣水等類型。

從氫氣濃度分析看,純凈水、自來水幾乎沒有氫氣,普通商品化氫氣水氫氣濃度非常低,這也符合日本市場特點,大部分氫水中氫氣濃度非常低,本次研究中的氫水氫氣濃度為0.075 ppm, ORP+ 49 mV,這種濃度大約為飽和濃度的1/20。

研究中的工業化氫氣水,我個人理解是剛剛生產出沒有經過運輸上市的氫水,這種氫水中氫氣的濃度可以達到0.788 ppm, ORP? 614 mV)這是比較符合情況的,這種濃度大約相當于飽和度的50%。他們的主要研究目標,氫氣納米氣泡水,氫氣形成納米氣泡中54%大小小于717納米(或者說是0.717微米),這是相對比較大的氣泡,納米氣泡氫氣水中氫氣濃度稍微低于工業生產的氫氣水。

而國內的納米氫氣水生產的氫氣水濃度遠遠高于日本這一文章所報道的數據。

研究采用的抗氧化能力檢測,一種是順磁共振技術,這種技術是檢測自由基的最高端技術,順磁共振本質上是檢測電子自旋產生的磁場,因為自由基存在不成對電子,所以可以產生信號,而傳統的磁共振信號是核自旋形成的磁場。一般醫院中的MRI原理都是檢測的核磁共振信號,與順磁共振不一樣。

另外一種2、2聯吡啶氧化還原分光光度法,2、2聯吡啶這是一種氧化還原指示劑,是一種白色或淺紅色結晶性粉末,易溶于醇、醚、苯、三氯甲烷和石油醚。可溶于水,溶液遇亞鐵鹽則顯紅色。兩種研究方法都顯示,雖然納米氣泡氫氣水中氫氣的濃度比工業生產的氫水低,但是其抗羥基自由基的能力更高,這一研究說明,納米氣泡氫氣水具有更理想的抗氧化效果,可能具有更好的商業前景。

本論文通信作者Nobuhiko Miwa彥美和,聯系方法

E-mail:[email protected],

TEL:+81-072-260-0088,

FAX:+81-072-260-0011

ShinyaKato, Daigo Matsuok, Nobuhiko Miw,

Antioxidant activities of nano-bubble hydrogen-dissolved water assessed by ESR a.pdf

Laboratoryof Cell-Death Control BioTechnology, Faculty of Life and EnvironmentalSciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara,Hiroshima 727–0023,Japan.

We prepared nano-bubble hydrogen-dissolvedwater (nano-H water) which contained hydrogen nano-bubblesof < 717-nm diameter for 54% of total bubbles. In DMPO-spin trapelectron spin resonance (ESR) method, the DMPO-OH : MnO ratio, being attributedto amounts of hydroxyl radicals (·OH), was 2.78 for pure water (dissolvedhydrogen [DH] = < 0.01 ppm, oxidation-reduction potential[ORP] = + 324 mV), 2.73 for tap water (0.01 ppm,+ 286 mV), 2.93 for commercially available hydrogen water(0.075 ppm, + 49 mV), and 2.66 for manufactured hydrogen water(0.788 ppm, ? 614 mV), whereas the nano-H water (0.678 ppm,? 644 mV) exhibited 2.05, showing the superiority of nano-H water toother types of hydrogen water in terms of · OH-scavenging activity.Then, reduction activity of nano-H water was assessed spectrophotometrically by2,2’-bipyridyl method. Differential absorbance at 530 nm was in the order:0.018 for pure water, 0.055 for tap water, 0.079 for nano-H water, 0.085 forcommercially available hydrogen water, and 0.090 for manufactured hydrogenwater, indicating a prominent reduction activity of hydrogen water and nano-Hwater against oxidation in ascorbate-coupled ferric ion-bipyridyl reaction.Thus, the nano-H water has an improved antioxidant activity as compared to hydrogenwater of the similar DH-level, indicating the more marked importance ofnano-bubbles rather than concentration of hydrogen in termsof · OH-scavenging.

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